Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3360
Título: Multiplexed electrochemical immunosensor for detection of celiac disease serological markers
Autor: Neves, Marta M. P. S.
González-García, María Begoña
Delerue-Matos, Cristina
Costa-García, Agustín
Palavras-chave: Celiac disease
Tissue transglutaminase
Gliadin
Electrochemical immunosensors
Nanomaterials
Multiplexed detection
Data: 2013
Editora: Elsevier
Relatório da Série N.º: Sensors and Actuators B: Chemical; Vol. 187
Resumo: Celiac disease (CD) is a gluten-induced autoimmune enteropathy characterized by the presence of antibodies against gliadin (AGA) and anti-tissue transglutaminase (anti-tTG) antibodies. A disposable electrochemical dual immunosensor for the simultaneous detection of IgA and IgG type AGA and antitTG antibodies in real patient’s samples is presented. The proposed immunosensor is based on a dual screen-printed carbon electrode, with two working electrodes, nanostructured with a carbon–metal hybrid system that worked as the transducer surface. The immunosensing strategy consisted of the immobilization of gliadin and tTG (i.e. CD specific antigens) on the nanostructured electrode surface. The electrochemical detection of the human antibodies present in the assayed serum samples was carried out through the antigen–antibody interaction and recorded using alkaline phosphatase labelled anti-human antibodies and a mixture of 3-indoxyl phosphate with silver ions was used as the substrate. The analytical signal was based on the anodic redissolution of enzymatically generated silver by cyclic voltammetry. The results obtained were corroborated with commercial ELISA kits indicating that the developed sensor can be a good alternative to the traditional methods allowing a decentralization of the analyses towards a point-of-care strategy.
Peer review: yes
URI: http://hdl.handle.net/10400.22/3360
ISSN: 0925-4005
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0925400512009318
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