Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3346
Título: Molinate quantification in environmental water by a glutathione-S-transferase based biosensor
Autor: Oliveira, Túlio I. S.
Oliveira, Marcela
Viswanathan, Subramanian
Barroso, M. Fátima
Barreiros, Luísa
Nunes, O. C.
Rodrigues, José A.
Lima-Neto, Pedro de
Mazzetto, Selma E.
Morais, Simone
Delerue-Matos, Cristina
Palavras-chave: Glutathione-S-transferase
Enzymatic biosensor
Voltammetry
Molinate
Rice paddy field floodwater
Data: 2013
Editora: Elsevier
Relatório da Série N.º: Talanta; Vol. 106
Resumo: A glutathione-S-transferase (GST)based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water.The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane–glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mgL -1 and presenting a detection limit of 0.064 mgL- 1. The developed biosensor is stable,and reusable during 15 days.The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements.
Peer review: yes
URI: http://hdl.handle.net/10400.22/3346
ISSN: 0039-9140
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0039914012009058#
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