Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3343
Título: Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7–10)
Autor: Amaral, I. F.
Neiva, I.
Silva, F. Ferreira da
Sousa, Susana
Piloto, A. M.
Lopes, C. D. F.
Barbosa, Mário A.
Kirkpatrick, C. J.
Pêgo, A. P.
Palavras-chave: Three-dimensional scaffolds
Surface grafting
Protein radiolabelling
Protein conformation
Spinal cord injury
Data: 2013
Editora: Elsevier
Relatório da Série N.º: Acta Biomaterialia; Vol. 9, Issue 3
Resumo: The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4% and 15%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII7–10). Immobilized rhFNIII7–10 was characterized in terms of amount (125I-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII7–10 with rhFNIII7–10 concentration, and, for the same concentration, higher amounts of rhFNIII7–10 on DA 4% compared with DA 15%. Moreover, rhFNIII7–10 concentrations as low as 5 and 20 lgml 1 in the coupling reaction were shown to provide DA 4% and 15% scaffolds, respectively, with levels of exposed cell-binding domains exceeding those observed on the control (DA 4% scaffolds incubated in a 20 lgml 1 human fibronectin solution). These grafting conditions proved to be effective in mediating EC adhesion/cytoskeletal organization on CH with DA 4% and 15%, without affecting the endothelial angiogenic potential. rhFNIII7–10 grafting to CH could be a strategy of particular interest in tissue engineering applications requiring the use of endothelialized porous matrices with tunable degradation rates.
Peer review: yes
URI: http://hdl.handle.net/10400.22/3343
ISSN: 1742-7061/$
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S174270611200517X
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