Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3008
Título: Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment
Autor: Barroso, M. Fátima
de-los-Santos-Álvarez, N.
Lobo-Castañón, M. J.
Miranda-Ordieres, A. J.
Delerue-Matos, Cristina
Oliveira, M. Beatriz P. P.
Tuñón-Blanco, P.
Palavras-chave: NADH electrocatalysis
DNA damage
Antioxidant capacity
Ascorbic acid
Reactive oxygen species
Superoxide radical
Data: 2011
Editora: Elsevier
Relatório da Série N.º: Journal of Electroanalytical Chemistry; Vol. 659, Issue 1
Resumo: The integrity of DNA purine bases was herein used to evaluate the antioxidant capacity. Unlike other DNA-based antioxidant sensors reported so far, the damaging agent chosen was the O 2 radical enzymatically generated by the xanthine/xanthine oxidase system. An adenine-rich oligonucleotide was adsorbed on carbon paste electrodes and subjected to radical damage in the presence/absence of several antioxidant compounds. As a result, partial damage on DNA was observed. A minor product of the radical oxidation was identified by cyclic voltammetry as a diimine adenine derivative also formed during the electrochemical oxidation of adenine/guanine bases. The protective efficiency of several antioxidant compounds was evaluated after electrochemical oxidation of the remaining unoxidized adenine bases, by measuring the electrocatalytic current of NADH mediated by the adsorbed catalyst species generated. A comparison between O 2 and OH radicals as a source of DNA lesions and the scavenging efficiency of various antioxidant compounds against both of them is discussed. Finally, the antioxidant capacity of beverages was evaluated and compared with the results obtained with an optical method.
Peer review: yes
URI: http://hdl.handle.net/10400.22/3008
ISSN: 1572-6657
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S1572665711002104
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