Name: | Description: | Size: | Format: | |
---|---|---|---|---|
95.84 KB | Adobe PDF |
Advisor(s)
Abstract(s)
A flow-spectrophotometric method is proposed for the routine determination of tartaric acid in wines. The
reaction between tartaric acid and vanadate in acetic media is carried out in flowing conditions and the subsequent colored
complex is monitored at 475 nm. The stability of the complex and the corresponding formation constant are presented.
The effect of wavelength and pH was evaluated by batch experiments. The selected conditions were transposed to a flowinjection
analytical system. Optimization of several flow parameters such as reactor lengths, flow-rate and injection
volume was carried out. Using optimized conditions, a linear behavior was observed up to 1000 µg mL-1 tartaric acid,
with a molar extinction coefficient of 450 L mg-1 cm-1 and ± 1 % repeatability. Sample throughput was 25 samples per
hour. The flow-spectrophotometric method was satisfactorily applied to the quantification of tartaric acid (TA) in wines
from different sources. Its accuracy was confirmed by statistical comparison to the conventional Rebelein procedure and
to a certified analytical method carried out in a routine laboratory.
Description
Keywords
Spectrophotometry Tartrate Wine Flow injection analysis
Citation
Publisher
Bentham Science