Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2901
Título: Gold electrode modified by self-assembled monolayers of thiols to determine DNA sequences hybridization
Autor: Silva, Mízia
Cavalcanti, Igor T.
Barroso, M. Fátima
Sales, M. Goreti F.
Dutra, Rosa Fireman
Palavras-chave: Single-stranded DNA
Genosensor
Self-assembled monolayer
Gold functionalized electrode
Data: 2010
Editora: Indian Academy of Sciences. Springer
Relatório da Série N.º: Journal of Chemical Sciences; Vol. 122, Issue 6
Resumo: The process of immobilization of biological molecules is one of the most important steps in the construction of a biosensor. In the case of DNA, the way it exposes its bases can result in electrochemical signals to acceptable levels. The use of self-assembled monolayer that allows a connection to the gold thiol group and DNA binding to an aldehydic ligand resulted in the possibility of determining DNA hybridization. Immobilized single strand of DNA (ssDNA) from calf thymus pre-formed from alkanethiol film was formed by incubating a solution of 2-aminoethanothiol (Cys) followed by glutaraldehyde (Glu). Cyclic voltammetry (CV) was used to characterize the self-assembled monolayer on the gold electrode and, also, to study the immobilization of ssDNA probe and hybridization with the complementary sequence (target ssDNA). The ssDNA probe presents a well-defined oxidation peak at +0.158 V. When the hybridization occurs, this peak disappears which confirms the efficacy of the annealing and the DNA double helix performing without the presence of electroactive indicators. The use of SAM resulted in a stable immobilization of the ssDNA probe, enabling the hybridization detection without labels. This study represents a promising approach for molecular biosensor with sensible and reproducible results.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2901
Versão do Editor: http://link.springer.com/article/10.1007%2Fs12039-010-0079-7
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