Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2895
Título: Reduced fluoresceinamine as a fluorescent sensor for nitric oxide
Autor: Duarte, Abel J.
Silva, Joaquim C. G. Esteves da
Palavras-chave: Reduced fluoresceinamine
NO sensor
Fiber optics
Data: 2010
Editora: Sensors
Resumo: A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10–750 µM with a limit of detection (LOD) of about 1 µM. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2895
ISSN: 1424-8220
Versão do Editor: http://www.mdpi.com/1424-8220/10/3/1661
Aparece nas colecções:ISEP – GRAQ – Artigos

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