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Advisor(s)
Abstract(s)
QuEChERS method was evaluated for extraction of 16 PAHs from fish samples. For a
selective measurement of the compounds, extracts were analysed by LC with fluorescence
detection. The overall analytical procedure was validated by systematic recovery
experiments at three levels and by using the standard reference material SRM
2977 (mussel tissue). The targeted contaminants, except naphthalene and acenaphthene,
were successfully extracted from SRM 2977 with recoveries ranging from
63.5–110.0% with variation coefficients not exceeding 8%. The optimum QuEChERS
conditions were the following: 5 g of homogenised fish sample, 10 mL of ACN, agitation
performed by vortex during 3 min. Quantification limits ranging from 0.12–
1.90 ng/g wet weight (0.30–4.70 µg/L) were obtained. The optimized methodology
was applied to assess the safety concerning PAHs contents of horse mackerel (Trachurus
trachurus), chub mackerel (Scomber japonicus), sardine (Sardina pilchardus) and
farmed seabass (Dicentrarchus labrax). Although benzo(a)pyrene, the marker used for
evaluating the carcinogenic risk of PAHs in food, was not detected in the analysed
samples (89 individuals corresponding to 27 homogenized samples), the overall
mean concentration ranged from 2.52 l 1.20 ng/g in horse mackerel to 14.6 ± 2.8 ng/
g in farmed seabass. Significant differences were found between the mean PAHs concentrations
of the four groups.
Description
Keywords
Fish Liquid chromatography Polycyclic aromatic hydrocarbons QuEChERS
Citation
Publisher
WILEY-VCH Verlag GmbH & Co.