Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2183
Título: Optimization and validation of organochlorine compounds in adipose tissue by SPE-gas chromatography
Autor: Fernandes, Virgínia C.
Pestana, Diogo
Monteiro, Rosário
Faria, Gil
Meireles, Manuela
Correia-Sá, Luísa
Teixeira, Diana
Faria, Ana
Calhau, Conceição
Domingues, Valentina F.
Delerue-Matos, Cristina
Palavras-chave: Organochlorine pesticides
TCDD
SPE
GC-ECD
GC-MS/MS
Adipose tissue
Data: 2012
Editora: John Wiley & Sons, Inc.
Relatório da Série N.º: Biomedical Chromatography; Vol. 26, Issue 12
Resumo: Scientific evidence has shown an association between organochlorine compounds (OCC) exposure and human health hazards. Concerning this, OCC detection in human adipose samples has to be considered a public health priority. This study evaluated the efficacy of various solid-phase extraction (SPE) and cleanup methods for OCC determination in human adipose tissue. Octadecylsilyl endcapped (C18-E), benzenesulfonic acid modified silica cation exchanger (SA), poly (styrene-divinylbenzene (EN) and EN/RP18 SPE sorbents were evaluated. The relative sample cleanup provided by these SPE columns was evaluated using gas chromatography with electron capture detection (GC–ECD). The C18-E columns with strong homogenization were found to provide the most effective cleanup, removing the greatest amount of interfering substance, and simultaneously ensuring good analyte recoveries higher than 70%. Recoveries>70% with standard deviations (SD)<15% were obtained for all compounds under the selected conditions. Method detection limits were in the 0.003–0.009 mg/kg range. The positive samples were confirmed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The highest percentage found of the OCC in real samples corresponded to HCB, o,p′-DDT and methoxychlor, which were detected in 80 and 95% of samples analyzed respectively. Copyright © 2012 John Wiley & Sons, Ltd.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2183
ISSN: 0269-3879
Versão do Editor: The definitive version is available at www3.interscience.wiley.com | http://onlinelibrary.wiley.com/doi/10.1002/bmc.2723/abstract;jsessionid=AFE62E8AFD42501F59ED9A90C9F0F28C.f03t02
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