Name: | Description: | Size: | Format: | |
---|---|---|---|---|
96.21 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Scientific evidence has shown an association between organochlorine compounds (OCC) exposure and human
health hazards. Concerning this, OCC detection in human adipose samples has to be considered a public health priority.
This study evaluated the efficacy of various solid-phase extraction (SPE) and cleanup methods for OCC determination in
human adipose tissue. Octadecylsilyl endcapped (C18-E), benzenesulfonic acid modified silica cation exchanger (SA), poly
(styrene-divinylbenzene (EN) and EN/RP18 SPE sorbents were evaluated. The relative sample cleanup provided by these
SPE columns was evaluated using gas chromatography with electron capture detection (GC–ECD). The C18-E columns with
strong homogenization were found to provide the most effective cleanup, removing the greatest amount of interfering
substance, and simultaneously ensuring good analyte recoveries higher than 70%. Recoveries>70% with standard
deviations (SD)<15% were obtained for all compounds under the selected conditions. Method detection limits were in
the 0.003–0.009 mg/kg range. The positive samples were confirmed by gas chromatography coupled with tandem mass
spectrometry (GC-MS/MS). The highest percentage found of the OCC in real samples corresponded to HCB, o,p′-DDT and
methoxychlor, which were detected in 80 and 95% of samples analyzed respectively. Copyright © 2012 John Wiley & Sons, Ltd.
Description
Keywords
Organochlorine pesticides TCDD SPE GC-ECD GC-MS/MS Adipose tissue
Citation
Publisher
John Wiley & Sons, Inc.