Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2037
Título: Analysis of polycyclic aromatic hydrocarbons in fish: optimisation and validation of microwave-assisted extraction
Autor: Ramalhosa, Maria João
Paíga, Paula
Morais, Simone
Sousa, Ana M. M.
Gonçalves, Maria Pilar
Delerue-Matos, Cristina
Oliveira, M. Beatriz P. P.
Palavras-chave: Polycyclic aromatic hydrocarbons
Microwave-assisted extraction
Response surface methodology
Liquid chromatography
Data: 2012
Editora: Elsevier
Relatório da Série N.º: Food Chemistry; Vol. 135, Issue 1
Resumo: An accurate and sensitive method for determination of 18 polycyclic aromatic hydrocarbons (PAHs) (16 PAHs considered by USEPA as priority pollutants, dibenzo[a,l]pyrene and benzo[j]fluoranthene) in fish samples was validated. Analysis was performed by microwave-assisted extraction and liquid chromatography with photodiode array and fluorescence detection. Response surface methodology was used to find the optimal extraction parameters. Validation of the overall methodology was performed by spiking assays at four levels and using SRM 2977. Quantification limits ranging from 0.15–27.16 ng/g wet weight were obtained. The established method was applied in edible tissues of three commonly consumed and commercially valuable fish species (sardine, chub mackerel and horse mackerel) originated from Atlantic Ocean. Variable levels of naphthalene (1.03–2.95 ng/g wet weight), fluorene (0.34–1.09 ng/g wet weight) and phenanthrene (0.34–3.54 ng/g wet weight) were detected in the analysed samples. None of the samples contained detectable amounts of benzo[a]pyrene, the marker used for evaluating the occurrence and carcinogenic effects of PAHs in food.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2037
ISSN: 0308-8146
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0308814612007418
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