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Ultrasensitive detection of ovarian cancer marker using immunoliposomes and gold nanoelectrodes
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Orientador(es)
Resumo(s)
Mucin-16 (MUC16) is the established ovarian cancer marker used to follow the disease during or after
treatment for epithelial ovarian cancer. The emerging science of cancer markers also demands for the
new sensitive detection methods. In this work, we have developed an electrochemical immunosensor
for antigen MUC16 using gold nanoelectrode ensemble (GNEE) and ferrocene carboxylic acid encapsulated
liposomes tethered with monoclonal anti-Mucin-16 antibodies ( MUC16). GNEEs were fabricated
by electroless deposition of the gold within the pores of polycarbonate track-etched membranes. Afterwards,
MUC16 were immobilized on preformed self-assembled monolayer of cysteamine on the GNEE
via cross-linking with EDC-Sulfo-NHS. A sandwich immunoassay was performed on MUC16 functionalized
GNEE with MUC16 and immunoliposomes. The differential pulse voltammetry was employed to
quantify the faradic redox response of ferrocene carboxylic acid released from immunoliposomes. The
dose–response curve for MUC16 concentration was found between the range of 0.001–300 U mL−1. The
lowest detection limit was found to be 5
× 10−4 U mL−1 (S/N = 3). We evaluated the performance of this
developed immunosensor with commercial ELISA assay by comparing results obtained from spiked serum
samples and real blood serum samples from volunteers.
Descrição
Palavras-chave
Immunosensor Ovarian cancer Mucin-16 Gold nanoelectrodes Liposome
Contexto Educativo
Citação
Editora
Elsevier