Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2022
Título: Voltammetric immunosensor for the diagnosis of celiac disease based on the quantification of anti-gliadin antibodies
Autor: Neves, Marta M. P. S.
González-García, María Begoña
Santos-Silva, Alice
Costa-García, Agustín
Palavras-chave: Gliadin
Celiac disease
Electrochemical immunosensors
Point-of-care analysis
Screen-printed electrodes
Nanomaterials
Data: 2012
Editora: Elsevier
Relatório da Série N.º: Sensors and Actuators B: Chemical; Vol.163, Issue 1
Resumo: Antibodies against gliadin are used to detect celiac disease (CD) in patients. An electrochemical immunosensor for the voltammetric detection of human anti-gliadin antibodies (AGA) IgA and AGA IgG in real serum samples is proposed. The transducer surface consists of screen-printed carbon electrodes modified with a carbon nanotube/gold nanoparticle hybrid system, which provides a very useful surface for the amplification of the immunological interactions. The immunosensing strategy is based on the immobilization of gliadin, the antigen for the autoantibodies of interest, onto the nanostructured surface. The antigen–antibody interaction is recorded using alkaline phosphatase labeled anti-human antibodies and a mixture of 3-indoxyl phosphate with silver ions (3-IP/Ag+) was used as the substrate. The analytical signal is based on the anodic redissolution of the enzymatically generated silver by cyclic voltammetry. The electrochemical behavior of this immunosensor was carefully evaluated assessing aspects as sensitivity, non-specific binding and matrix effects, and repeatability and reproducibility. The results were supported with a commercial ELISA test.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2022
ISSN: 0925-4005
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0925400512000718
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