Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2013
Título: Celiac disease detection using a transglutaminase electrochemical immunosensor fabricated on nanohybrid screen-printed carbon electrodes
Autor: Neves, Marta M. P. S.
González-García, María Begoña
Nouws, Henri P. A.
Costa-García, Agustín
Palavras-chave: Transglutaminase
Celiac disease
Screen-printed electrodes
Carbon nanotubes
Gold nanoparticles
Data: 2012
Editora: Elsevier
Relatório da Série N.º: Biosensors and Bioelectronics; Vol. 31, Issue 1
Resumo: Celiac disease is a gluten-induced autoimmune enteropathy characterized by the presence of tissue tranglutaminase (tTG) autoantibodies. A disposable electrochemical immunosensor (EI) for the detection of IgA and IgG type anti-tTG autoantibodies in real patient’s samples is presented. Screen-printed carbon electrodes (SPCE) nanostructurized with carbon nanotubes and gold nanoparticles were used as the transducer surface. This transducer exhibits the excellent characteristics of carbon–metal nanoparticle hybrid conjugation and led to the amplification of the immunological interaction. The immunosensing strategy consisted of the immobilization of tTG on the nanostructured electrode surface followed by the electrochemical detection of the autoantibodies present in the samples using an alkaline phosphatase (AP) labelled anti-human IgA or IgG antibody. The analytical signal was based on the anodic redissolution of enzymatically generated silver by cyclic voltammetry. The results obtained were corroborated with a commercial ELISA kit indicating that the electrochemical immunosensor is a trustful analytical screening tool.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2013
ISSN: 0956-5663
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0956566311006658
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