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|Título:||Iron oxide/gold core/shell nanomagnetic probes and CdS biolabels for amplified electrochemical immunosensing of Salmonella typhimurium|
Nouws, Henri P. A.
Oliveira, M. Beatriz P. P.
|Relatório da Série N.º:||Biosensors and Bioelectronics; Vol. 51|
|Resumo:||There is an imminent need for rapid methods to detect and determine pathogenic bacteria in food products as alternatives to the laborious and time-consuming culture procedures. In this work, an electrochemical immunoassay using iron/gold core/shell nanoparticles (Fe@Au) conjugated with anti-Salmonella antibodies was developed. The chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles is reported. Fe@Au nanoparticles were functionalized with different self-assembled monolayers and characterized using ultraviolet-visible spectrometry, transmission electron microscopy, and voltammetric techniques. The determination of Salmonella typhimurium, on screen-printed carbon electrodes, was performed by square-wave anodic stripping voltammetry through the use of CdS nanocrystals. The calibration curve was established between 1×101 and 1×106 cells/mL and the limit of detection was 13 cells/mL. The developed method showed that it is possible to determine the bacteria in milk at low concentrations and is suitable for the rapid (less than 1 h) and sensitive detection of S. typhimurium in real samples. Therefore, the developed methodology could contribute to the improvement of the quality control of food samples.|
|Versão do Editor:||http://www.sciencedirect.com/science/article/pii/S0956566313005204|
|Aparece nas colecções:||ISEP – GRAQ – Artigos|
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|ART_MariaFreitas_2014_GRAQ.pdf||95,84 kB||Adobe PDF||Ver/Abrir|
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