Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/2012
Título: Iron oxide/gold core/shell nanomagnetic probes and CdS biolabels for amplified electrochemical immunosensing of Salmonella typhimurium
Autor: Freitas, Maria
Viswanathan, Subramanian
Nouws, Henri P. A.
Oliveira, M. Beatriz P. P.
Delerue-Matos, Cristina
Palavras-chave: Salmonella typhimurium
Electrochemical immunoassay
Magnetic nanoparticles
Cadmium nanocrystals
Labeled detection
Data: 2014
Editora: Elsevier
Relatório da Série N.º: Biosensors and Bioelectronics; Vol. 51
Resumo: There is an imminent need for rapid methods to detect and determine pathogenic bacteria in food products as alternatives to the laborious and time-consuming culture procedures. In this work, an electrochemical immunoassay using iron/gold core/shell nanoparticles (Fe@Au) conjugated with anti-Salmonella antibodies was developed. The chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles is reported. Fe@Au nanoparticles were functionalized with different self-assembled monolayers and characterized using ultraviolet-visible spectrometry, transmission electron microscopy, and voltammetric techniques. The determination of Salmonella typhimurium, on screen-printed carbon electrodes, was performed by square-wave anodic stripping voltammetry through the use of CdS nanocrystals. The calibration curve was established between 1×101 and 1×106 cells/mL and the limit of detection was 13 cells/mL. The developed method showed that it is possible to determine the bacteria in milk at low concentrations and is suitable for the rapid (less than 1 h) and sensitive detection of S. typhimurium in real samples. Therefore, the developed methodology could contribute to the improvement of the quality control of food samples.
Peer review: yes
URI: http://hdl.handle.net/10400.22/2012
ISSN: 0956-5663
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0956566313005204
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